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Recombinant Human DHODH protein, GST-tagged

Cat.No. : DHODH-11970H
Product Overview : Recombinant Human DHODH (1-395aa) protein was fused to GST-tag and expressed in E.coli.
  • Specification
  • Gene Information
  • Related Products
Description : The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
Source : E.coli
Species : Human
Tag : GST
Form : The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Molecular Mass : 66 kDa
Protein length : 395aa
AA Sequence : MAWRHLKKRAQDAVIILGGGGLLFA SYLMATGDERFYAEHLMPTLQGLLD PESAHRLAVRFTSLGLLPRARFQDS DMLEVRVLGHKFRNPVGIAAGFDKH GEAVDGLYKMGFGFVEIGSVTPKPQ EGNPRPRVFRLPEDQAVINRYGFNS HGLSVVEHRLRARQQKQAKLTEDGL PLGVNLGKNKTSVDAAEDYAEGVRV LGPLADYLVVNVSSPNTAGLRSLQG KAELRRLLTKVLQERDGLRRVHRPA VLVKIAPDLTSQDKEDIASVVKELG IDGLIVTNTTVSRPAGLQGALRSET GGLSGKPLRDLSTQTIREMYALTQG RVPIIGVGGVSSGQDALEKIRAGAS LVQLYTALTFWGPPVVGKVKRELEA LLKEQGFGGVTDAIGADHRR
Purity : 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Storage : Short-term storage: Store at 2-8 centigrade for (1-2 weeks).
Long-term storage: Aliquot and store at -20 to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Reconstitution : Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : DHODH
Official Symbol : DHODH
Synonyms : URA1; POADS; DHOdehase
Gene ID : 1723
mRNA Refseq : NM_001361.5
Protein Refseq : NP_001352.2
MIM : 126064
UniProt ID : Q02127

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does DHODH contribute to pyrimidine biosynthesis? 08/28/2022

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism? 10/23/2020

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway? 10/27/2018

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth? 05/07/2018

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis? 12/29/2017

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases? 08/01/2017

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment? 07/17/2017

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.

Customer Reviews (3)

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Reviews
07/29/2020

    Saves time and effort. Highly recommended for research.

    11/03/2017

      Top-notch support for insights. Trusted for accurate data.

      05/05/2017

        Reliable data, essential for our protein research.

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