Recombinant Human Di-Ubiquitin Protein (DUB-Resistant K48-Linked)

• Cat.No.: Di-Ubiquitin-38H
• Product Overview: Recombinant Human Di-Ubiquitin Protein (DUB-Resistant K48-Linked) without tag was expressed in E. coli. The product contains a K48R mutation in the distal ubiquitin and a wild-type C-terminus on the proximal ubiquitin.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: DUB-resistant, K48-linked Di-ubiquitin (Ub2) contains an amino acid substitution near the C-terminus of the distal ubiquitin that renders it resistant to deubiquitylases (DUBs). DUB-resistant ubiquitin chains are ideal reagents for structural or binding studies with deubiquitylases or other ubiquitin binding proteins. They can also be used as inhibitors or to determine the linkage-specificity of DUBs.
• Molecular Mass: 17,123.7 Da
• Purity: ≥ 90% by RP-HPLC
• Applications: Structural or binding studies with deubiquitylases and other ubiquitin binding proteins. Inhibition or determination of linkage-specificity of DUBs
• Storage: At -80 centigrade, avoid freeze/thaw cycles
• Concentration: 1 mg/mL
• Storage Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA

Gene Information

• Official Symbol: Di-Ubiquitin
• Synonyms: Di-Ubiquitin; Ub2