Recombinant Human Diablo Homolog (Drosophila), T7-tagged

• Cat.No.: DIABLO-377H
• Product Overview: Recombinant human Smac fused to T7-tag. at N-terminus was expressed in E.coli and purified by conventional chromatography techniques, 22 kDa (199 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: Smac/Diablo is one of the proapoptotic proteins. Smac promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity.
• Sequences of amino acids: MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5)
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DIABLO diablo homolog (Drosophila) [ Homo sapiens ]
• Synonyms: DIABLO; diablo homolog (Drosophila); SMAC; SMAC3; DIABLO-S; FLJ10537; FLJ25049;Diablo; 0610041G12Rik; mitochondrial Smac protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; 5` partial; Diablo homolog, mitochondrial; diablo; Direct IAP-binding protein with low Pi; Second mitochondria-derived activator of caspase; Smac protein
• Gene ID: 56616
• mRNA Refseq: NM_019887
• Protein Refseq: NP_063940
• MIM: 605219
• UniProt ID: Q9NR28
• Chromosome Location: 12q24
• Pathway: Apoptosis
• Function: protein binding