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Active Recombinant Human DKK1 Protein, Biotinylated

Cat.No. : DKK1-164H
Product Overview : Recombinant Human DKK1(Thr32-His266) was expressed in Insect cells, Biotinylated.
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Description : Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains (CRDs) separated by a linker region. Each domain contains ten cysteine residues. Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 83% amino acid sequence identity with mouse and rat Dkk-1. Dkk-1 is a well-characterized antagonist of Wnt/beta-Catenin signaling. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP-5 or LRP-6. Crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously. Dkk-1/LRP-6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles, and the retina. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death. Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis.
Source : Insect cells
Species : Human
Predicted N Terminal : Thr32
Form : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : easured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.2-1 μg/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a.
Molecular Mass : Predicted Molecular Mass: 26 kDa (unlabeled);
SDS-PAGE: 33-38 kDa, reducing conditions.
Protein length : Thr32-His266
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE with silver staining
Notes : Structure / Form: Monomer, biotinylated via sugars
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile
Reconstitution : Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
Official Symbol : DKK1
Synonyms : DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1;
Gene ID : 22943
mRNA Refseq : NM_012242
Protein Refseq : NP_036374
MIM : 605189
UniProt ID : O94907

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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