|Product Overview:||Recombinant human DKK-3 (Ala22-Ile350) protein with a C-terminal 10-His tag was expressed in NS0 cells.|
|Bio-activity:||Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Hsieh, S.Y. et al. (2004) Oncogene 23:9183. The ED50 for this effect is 3-12 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 37.5 kDa
SDS-PAGE: 75-80 kDa, reducing conditions
|N-terminal Sequence Analysis:||Ala22|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution:||Reconstitute at 250 μg/mL in sterile PBS.|
|Official Symbol:||DKK3 dickkopf WNT signaling pathway inhibitor 3 [ Homo sapiens (human) ]|
|Synonyms:||dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1; DKK3; dickkopf WNT signaling pathway inhibitor 3|