Active Recombinant Human DLL4 protein, Fc-tagged, Low Endotoxin

• Cat.No.: DLL4-686H
• Product Overview: Recombinant Human DLL4 protein(Q9NR61)(Ser27-Pro524), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 27-524 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Notch 1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DLL4 delta-like 4 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL4
• Synonyms: DLL4; delta-like 4 (Drosophila); delta like 4 homolog (Drosophila); delta-like protein 4; delta4; delta 4; delta ligand 4; notch ligand DLL4; delta-like 4 homolog; delta-like 4 protein; notch ligand delta-2; drosophila Delta homolog 4; hdelta2; MGC126344
• Gene ID: 54567
• mRNA Refseq: NM_019074
• Protein Refseq: NP_061947
• MIM: 605185
• UniProt ID: Q9NR61