Recombinant Human DNASE1 Protein (Gly19-Ala259), His tagged

• Cat.No.: DNASE1-1974H
• Product Overview: Recombinant human DNASE1 (Gly19-Ala259) fused with His tag was expressed in E. coli.

Specification

• Description: Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 27.9 kDa
• Protein Length: Gly19-Ala259
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Homo sapiens (human) ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease I; DNL1; deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I; DRNI; FLJ38093; FLJ44902; DKFZp686H0155
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855