Active Recombinant Full Lenght Human DNMT1 Protein, His&Flag tagged

• Cat.No.: DNMT1-388H
• Product Overview: Full length recombinant DNMT1 protein (accession number NP_001370.1) was expressed in Sf9 cells and contains an N-terminal 6×His Tag and FLAG-Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Protein Length: 1-1616 aa
• Description: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
• Form: 25 mM HEPES-NaOH pH 7.5, 500 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.
• Molecular Mass: 188.2 kDa
• Purity: > 90% by SDS-PAGE
• Applications: Recombinant DNMT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity/Inhibition Assay for 1 hour at 37 centigrade.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Gene Information

• Gene Name: DNMT1 DNA methyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA methyltransferase 1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; DNA MTase HsaI; DNA methyltransferase HsaI; EC 2.1.1.37
• Gene ID: 1786
• mRNA Refseq: NM_001379
• Protein Refseq: NP_001370
• MIM: 126375
• UniProt ID: P26358