Recombinant Human DOK1 Protein (1-481 aa), His-SUMO-tagged

• Cat.No.: DOK1-1110H
• Product Overview: Recombinant Human DOK1 Protein (1-481 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cardiovascular. Protein Description: Full Length.

Specification

• Description: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assbly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 68.4 kDa
• Protein length: 1-481 aa
• AA Sequence: MDGAVMEGPLFLQSQRFGTKRWRKT WAVLYPASPHGVARLEFFDHKGSSS GGGRGSSRRLDCKVIRLAECVSVAP VTVETPPEPGATAFRLDTAQRSHLL AADAPSSAAWVQTLCRNAFPKGSWT LAPTDNPPKLSALEMLENSLYSPTW EGSQFWVTVQRTEAAERCGLHGSYV LRVEAERLTLLTVGAQSQILEPLLS WPYTLLRRYGRDKVMFSFEAGRRCP SGPGTFTFQTAQGNDIFQAVETAIH RQKAQGKAGQGHDVLRADSHEGEVA EGKLPSPPGPQELLDSPPALYAEPL DSLRIAPCPSQDSLYSDPLDSTSAQ AGEGVQRKKPLYWDLYEHAQQQLLK AKLTDPKEDPIYDEPEGLAPVPPQG LYDLPREPKDAWWCQARVKEEGYEL PYNPATDDYAVPPPRSTKPLLAPKP QGPAFPEPGTATGSGIKSHNSALYS QVQKSGASGSWDCGLSRVGTDKTGV KSEGST
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: DOK1 docking protein 1, 62kDa (downstream of tyrosine kinase 1) [ Homo sapiens ]
• Official Symbol: DOK1
• Synonyms: DOK1; docking protein 1; p62dok; pp62; p62(dok); P62DOK; MGC117395; MGC138860
• Gene ID: 1796
• mRNA Refseq: NM_001197260
• Protein Refseq: NP_001184189
• MIM: 602919
• UniProt ID: Q99704