Recombinant Human DPH1 Protein, Myc/DDK-tagged, C13 and N15-labeled
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| Cat.No. : | DPH1-2128H |
| Product Overview : | DPH1 MS Standard C13 and N15-labeled recombinant protein (NP_001374) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
| Description : | The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | Myc/DDK |
| Molecular Mass : | 48.6 kDa |
| AA Sequence : | MRRQVMAALV VSGAAEQGGR DGPGRGRAPR GRVANQIPPE ILKNPQLQAA IRVLPSNYNF EIPKTIWRIQ QAQAKKVALQ MPEGLLLFAC TIVDILERFT EAEVMVMGDV TYGACCVDDF TARALGADFL VHYGHSCLIP MDTSAQDFRV LYVFVDIRID TTHLLDSLRL TFPPATALAL VSTIQFVSTL QAAAQELKAE YRVSVPQCKP LSPGEILGCT SPRLSKEVEA VVYLGDGRFH LESVMIANPN VPAYRYDPYS KVLSREHYDH QRMQAARQEA IATARSAKSW GLILGTLGRQ GSPKILEHLE SRLRALGLSF VRLLLSEIFP SKLSLLPEVD VWVQVACPRL SIDWGTAFPK PLLTPYEAAV ALRDISWQQP YPMDFYAGSS LGPWTVNHGQ DRRPHAPGRP ARGKVQEGSA RPPSAVACED CSCRDEKVAP LAPTRTRPLE QKLISEEDLA ANDILDYKDD DDKV |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : | 50 μg/mL as determined by BCA |
| Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
| SDS-PAGE : |
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| Gene Name : | DPH1 diphthamide biosynthesis 1 [ Homo sapiens (human) ] |
| Official Symbol : | DPH1 |
| Synonyms : | DPH1; DPH1 homolog (S. cerevisiae); diptheria toxin resistance protein required for diphthamide biosynthesis like 1 (S. cerevisiae), DPH like 1 (S. cerevisiae), DPH2L, DPH2L1; diphthamide biosynthesis protein 1; ovarian tumor suppressor candidate 1; OVCA1; DPH-like 1; DPH2-like 1; ovarian cancer-associated gene 1 protein; candidate tumor suppressor in ovarian cancer 1; diphthamide biosynthesis protein 2 homolog-like 1; diptheria toxin resistance protein required for diphthamide biosynthesis-like 1; diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1; DPH2L; DPH2L1; FLJ33211; |
| Gene ID : | 1801 |
| mRNA Refseq : | NM_001383 |
| Protein Refseq : | NP_001374 |
| MIM : | 603527 |
| UniProt ID : | Q9BZG8 |
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