Recombinant Human DRAP1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: DRAP1-254H
• Product Overview: DRAP1 MS Standard C13 and N15-labeled recombinant protein (NP_006433) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 22.3 kDa
• AA Sequence: MPSKKKKYNARFPPARIKKIMQTDE EIGKVAAAVPVIISRALELFLESLL KKACQVTQSRNAKTMTTSHLKQCIE LEQQFDFLKDLVASVPDMQGDGEDN HMDGDKGARRGRKPGSGGRKNGGMG TKSKDKKLSGTDSEQEDESEDTDTD GEEETSQPPPQASHPSAHFQSPPTP FLPFASTLPLPPAPPGPSAPDEEDE EDYDSTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DRAP1 DR1-associated protein 1 (negative cofactor 2 alpha) [ Homo sapiens (human) ]
• Official Symbol: DRAP1
• Synonyms: DRAP1; DR1-associated protein 1 (negative cofactor 2 alpha); dr1-associated corepressor; DR1 associated corepressor; NC2 alpha; negative cofactor 2 alpha; negative co-factor 2-alpha; NC2-alpha
• Gene ID: 10589
• mRNA Refseq: NM_006442
• Protein Refseq: NP_006433
• MIM: 602289
• UniProt ID: Q14919