Recombinant Human DUS2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DUS2-3072H
• Product Overview: DUS2L MS Standard C13 and N15-labeled recombinant protein (NP_060273) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene.
• Molecular Mass: 55.1 kDa
• AA Sequence: MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DUS2 dihydrouridine synthase 2 [ Homo sapiens (human) ]
• Official Symbol: DUS2
• Synonyms: DUS2; dihydrouridine synthase 2; SMM1; DUS2L; URLC8; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; SMM1 homolog; dihydrouridine synthase 2-like, SMM1 homolog; tRNA-dihydrouridine(20) synthase (NAD(P)(+)); up-regulated in lung cancer protein 8; EC 1.3.1.91
• Gene ID: 54920
• mRNA Refseq: NM_017803
• Protein Refseq: NP_060273
• MIM: 609707
• UniProt ID: Q9NX74