Recombinant Human DUSP4, GST-tagged

• Cat.No.: DUSP4-101H
• Product Overview: Human DUSP4 full-length ORF ( AAH02671, 1 a.a. - 394 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 69.08 kDa
• AA Sequence: MVTMEELREMDCSVLKRLMNRDENG GGAGGSGSHGTLGLPSGGKCLLLDC RPFLAHSAGYILGSVNVRCNTIVRR RAKGSVSLEQILPAEEEVRARLRSG LYSAVIVYDERSPRAESLREDSTVS LVVQALRRNAERTDICLLKGGYERF SSEYPEFCSKTKALAAIPPPVPPSA TEPLDLGCSSCGTPLHDQGGPVEIL PFLYLGSAYHAARRDMLDALGITAL LNVSSDCPNHFEGHYQYKCIPVEDN HKADISSWFMEAIEYIDAVKDCRGR VLVHCQAGISRSATICLAYLMMKKR VRLEEAFEFVKQRRSIISPNFSFMG QLLQFESQVLATSCAAEAASPSGPL RERGKTPATPTSQFVFSFPVSVGVH SAPSSLPYLHSPITTSPSC
• Applications: ELISA; WB; Antibody Production; Protein Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DUSP4 dual specificity phosphatase 4 [ Homo sapiens (human) ]
• Official Symbol: DUSP4
• Synonyms: DUSP4; TYP; HVH2; MKP2; MKP-2; dual specificity phosphatase 4; MAP kinase phosphatase 2; VH1 homologous phosphatase 2; dual specificity protein phosphatase hVH2; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 2; EC 3.1.3.16; EC 3.1.3.48
• Gene ID: 1846
• mRNA Refseq: NM_001394
• Protein Refseq: NP_001385
• MIM: 602747
• UniProt ID: Q13115
• Chromosome Location: 8p12-p11
• Pathway: Activated TLR4 signalling; B Cell Receptor Signaling Pathway; ERK/MAPK targets
• Function: MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/threonine phosphatase activity