Recombinant Human E4F1 Protein, GST-tagged

• Cat.No.: E4F1-3011H
• Product Overview: Human E4F1 full-length ORF ( AAH80524.1, 1 a.a. - 784 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. [provided by RefSeq, Jan 2014]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 109.9 kDa
• AA Sequence: MEGAMAVRVTAAHTAEAQAEAGREA GEGAVAAVAAALAPSGFLGLPAPFS EEDEDDVHRCGRCQAEFTALEDFVQ HKIQKACQRAPPEALPATPATTALL GQEVVPAAPGPEEPITVAHIVVEAA SLAADISHASDLVGGGHIKEVIVAA EAELGDGEMAEAPGSPHQQGLGLAG EGEQAQVKLLVNKDGRYVCALCHKT FKTGSILKAHMVTHSSRKDHECKLC GASFRTKGSLIRHHRRHTDERPYKC SKCGKSFRESGALTRHLKSLTPCTE KIRFSVSKDVVVSKEDARAGSGAGA AGLGTATSSVTGEPIETSPVIHLVT DAKGTVIHEVHVQMQELSLGMKALA PEPPVSQELPCSSEGSRENLLHQAM QNSGIVLERAAGEEGALEPAPAAGS SPQPLAVAAPQLPVLEVQPLETQVA SEASAVPRTHPCPQCSETFPTAATL EAHKRGHTGPRPFACAQCGKAFPKA YLLKKHQEVHVRERRFRCGDCGKLY KTIAHVRGHRRVHSDERPYPCPKCG KRYKTKNAQQVHFRTHLEEKPHVCQ FCSRGFREKGSLVRHVRHHTGEKPF KCYKCGRGFAEHGTLNRHLRTKGGC LLEVEELLVSEDSPAAATTVLTEDP HTVLVEFSSVVADTQEYIIEATADD AETSEATEIIEGTQTEVDSHIMKVV QQIVHQASAGHQIIVQNVTMDEETA LGPEAAAADTITIATPESLTEQVAM TLASAISEGTVLAARAGTSGTEQAT VTMVSSEDIEILEHAGELVIASPEG QLEVQTVIV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: E4F1 E4F transcription factor 1 [ Homo sapiens ]
• Official Symbol: E4F1
• Synonyms: E4F1; E4F transcription factor 1; transcription factor E4F1; E4F; p50E4F; p120E4F; transcription factor E4F; putative E3 ubiquitin-protein ligase E4F1; MGC99614
• Gene ID: 1877
• mRNA Refseq: NM_004424
• Protein Refseq: NP_004415
• MIM: 603022
• UniProt ID: Q66K89