Active Recombinant Human EFNA4 protein, hFc-tagged, Low Endotoxin

• Cat.No.: EFNA4-177H
• Product Overview: Recombinant Human EFNA4 protein(P52798-1)(Leu26-Gly171), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 26-171 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EphA7, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 43.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EFNA4 ephrin-A4 [ Homo sapiens ]
• Official Symbol: EFNA4
• Synonyms: EFNA4; ephrin-A4; EPLG4; LERK4; LERK-4; ligand of eph-related kinase 4; eph-related receptor tyrosine kinase ligand 4; EFL4; FLJ57652; MGC125826
• Gene ID: 1945
• mRNA Refseq: NM_005227
• Protein Refseq: NP_005218
• MIM: 601380
• UniProt ID: P52798