Recombinant Human EFNA4 protein, His-tagged

• Cat.No.: EFNA4-323H
• Product Overview: Recombinant Human EFNA4 protein(P52798-1)(Leu26-Gly171), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu26-Gly171
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EFNA4 ephrin-A4 [ Homo sapiens ]
• Official Symbol: EFNA4
• Synonyms: EFNA4; ephrin-A4; EPLG4; LERK4; LERK-4; ligand of eph-related kinase 4; eph-related receptor tyrosine kinase ligand 4; EFL4; FLJ57652; MGC125826
• Gene ID: 1945
• mRNA Refseq: NM_005227
• Protein Refseq: NP_005218
• MIM: 601380
• UniProt ID: P52798