Recombinant Human EIF3A protein, His-tagged

• Cat.No.: EIF3A-3012H
• Product Overview: Recombinant Human EIF3A protein(266-437 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 266-437 aa
• AA Sequence: PPKPQLMANYYNKVSTVFWKSGNAL FHASTLHRLYHLSREMRKNLTQDEM QRMSTRVLLATLSIPITPERTDIAR LLDMDGIIVEKQRRLATLLGLQAPP TRIGLINDMVRFNVLQYVVPEVKDL YNWLEVEFNPLKLCERVTKVLNWVR EQPEKEPELQQYVPQLQNNTIL
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: EIF3A eukaryotic translation initiation factor 3, subunit A [ Homo sapiens ]
• Official Symbol: EIF3A
• Synonyms: EIF3A; eukaryotic translation initiation factor 3, subunit A; EIF3, EIF3S10, eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3 subunit A; eIF3 p170; eIF3 theta; eIF3a; KIAA0139; TIF32; eIF3 p167; eIF3 p180; eIF3 p185; eIF-3-theta; EIF3, p180 subunit; centrosomin homolog; cytoplasmic protein p167; eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3, subunit 10, 170kD; eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); EIF3; P167; p180; p185; EIF3S10; eIF3-p170; eIF3-theta
• Gene ID: 8661
• mRNA Refseq: NM_003750
• Protein Refseq: NP_003741
• MIM: 602039
• UniProt ID: Q14152