Recombinant Human EIF3I Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EIF3I-3218H
• Product Overview: EIF3I MS Standard C13 and N15-labeled recombinant protein (NP_003748) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 36.5 kDa
• AA Sequence: MKPILLQGHERSITQIKYNREGDLL FTVAKDPIVNVWYSVNGERLGTYMG HTGAVWCVDADWDTKHVLTGSADNS CRLWDCETGKQLALLKTNSAVRTCG FDFGGNIIMFSTDKQMGYQCFVSFF DLRDPSQIDNNEPYMKIPCNDSKIT SAVWGPLGECIIAGHESGELNQYSA KSGEVLVNVKEHSRQINDIQLSRDM TMFVTASKDNTAKLFDSTTLEHQKT FRTERPVNSAALSPNYDHVVLGGGQ EAMDVTTTSTRIGKFEARFFHLAFE EEFGRVKGHFGPINSVAFHPDGKSY SSGGEDGYVRIHYFDPQYFEFEFEA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EIF3I eukaryotic translation initiation factor 3 subunit I [ Homo sapiens (human) ]
• Official Symbol: EIF3I
• Synonyms: EIF3I; eukaryotic translation initiation factor 3, subunit I; EIF3S2, eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3 subunit I; eIF3 beta; eIF3 p36; eIF3i; TRIP 1; eIF-3-beta; predicted protein of HQ2242; TGFbeta receptor-interacting protein 1; TGF-beta receptor-interacting protein 1; eukaryotic translation initiation factor 3 subunit 2; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
• Gene ID: 8668
• mRNA Refseq: NM_003757
• Protein Refseq: NP_003748
• MIM: 603911
• UniProt ID: Q13347