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Recombinant Human EIF4G1 protein, His-tagged

Cat.No. : EIF4G1-12383H
Product Overview : Recombinant Human EIF4G1 protein(296-645 aa), fused wuth His tag, was expressed in E.coli.
Availability February 16, 2025
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Source : E.coli
Species : Human
Tag : His
Form : The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
Protein length : 296-645 aa
AA Sequence : IEEYLHLNDMKEAVQCVQELASPSL LFIFVRHGVESTLERSAIAREHMGQ LLHQLLCAGHLSTAQYYQGLYEILE LAEDMEIDIPHVWLYLAELVTPILQ EGGVPMGELFREITKPLRPLGKAAS LLLEILGLLCKSMGPKKVGTLWREA GLSWKEFLPEGQDIGAFVAEQKVEY TLGEESEAPGQRALPSEELNRQLEK LLKEGSSNQRVFDWIEANLSEQQIV SNTLVRALMTAVCYSAIIFETPLRV DVAVLKARAKLLQKYLCDEQKELQA LYALQALVVTLEQPPNLLRMFFDAL YDEDVVKEDAFYSWESSKDPAEQQG KGVALKSVTAFFKWLREAEEESDHN
Purity : 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Storage : Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
Reconstitution : Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.
Gene Name : EIF4G1 eukaryotic translation initiation factor 4 gamma 1 [ Homo sapiens (human) ]
Official Symbol : EIF4G1
Synonyms : EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Gene ID : 1981
mRNA Refseq : NM_004953
Protein Refseq : NP_004944
MIM : 600495
UniProt ID : Q04637

Not For Human Consumption!

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Customer Reviews (3)

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05/19/2021

    The activity is strong, and the protein requires only a small amount to achieve the desired effect compared to other proteins.

    01/29/2021

      Very suitable for biosensors, high sensitivity.

      10/07/2020

        It performs very well in terms of recombinant expression.

        Q&As (6)

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        Can EIF4G1 protein be used as a potential therapeutic target? 11/04/2019

        Some current findings suggest that EIF4G1 may be a potential target for cancer treatment and other disease treatments. However, research in this field is still relatively early, and more experimental and clinical validation is still needed.

        Does mutation in EIF4G1 affect its biological characteristics? 10/29/2019

        Mutations in the EIF4G1 protein may affect the assembly and functioning of its translation complex, thereby affecting its biological function in cell physiology and disease processes.

        What important cell biological processes is EIF4G1 protein involved in? 06/29/2019

        The protein is involved in important cell biological processes such as the formation of translational initiation complexes, mRNA translation, transcriptional regulation, and cell signaling.

        Is EIF4G1 protein involved in drug therapy? 05/22/2019

        Research on EIF4G1 protein versus drug therapy is still in its infancy, but there are already many studies that show that EIF4G1 protein is very important for drug treatment.

        What is the role of EIF4G1 protein in cancer? 04/23/2019

        Promotes cancer cell growth, proliferation, and deterioration by facilitating the assembly of transcriptional and translational initiation complexes. Therefore, the researchers now believe that EIF4G1 may be a potential target for cancer treatment.

        In which diseases is EIF4G1 protein useful in the diagnosis and treatment? 01/14/2019

        EIF4G1 plays an important role in the occurrence and progression of various diseases such as cancer, neurodegenerative diseases and inflammation, and its application in the treatment of these diseases is currently being actively explored.

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