Recombinant Human EME1 Protein (1-583 aa), His-SUMO-tagged

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Recombinant Human EME1 Protein (1-583 aa), His-SUMO-tagged

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Cat.No. : EME1-1106H
Product Overview : Recombinant Human EME1 Protein (1-583 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cell Biology. Protein Description: Full Length of Isoform 2.
Description : Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Source : E. coli
Species : Human
Tag : His-SUMO
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 80.8 kDa
Protein length : 1-583 aa
AA Sequence : MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS LVIVDQEKCF SLELLFFDFL PCTSAQNPPR RGKQGANKQT KKQQQRQPEA SIGSMVSRVD AEEALVDLQL HTEAQAQIVQ SWKELADFTC AFTKAVAEAP FKKLRDETTF SFCLESDWAG GVKVDLAGRG LALVWRRQIQ QLNRVSLEMA SAVVNAYPSP QLLVQAYQQC FSDKERQNLL ADIQVRRGEG VTSTSRRIGP ELSRRIYLQM TTLQPHLSLD SAD
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe) [ Homo sapiens ]
Official Symbol : EME1
Synonyms : EME1; FLJ31364; MMS4L; SLX2A; hMMS4; MMS4 homolog;
Gene ID : 146956
mRNA Refseq : NM_001166131
Protein Refseq : NP_001159603
MIM : 610885
UniProt ID : Q96AY2

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