Recombinant Human EPCAM Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: EPCAM-7818H
• Product Overview: Biotinylated Human EpCAM, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Predicted N Terminal: Gln 24
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 56.1 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Gln24-Lys265

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EPCAM
• Synonyms: EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422

Reviews

02/13/2022

Reliable for assays, satisfied customer.

09/09/2019

High-quality product, consistent results.

04/03/2019

Quick delivery, product as described.

Q&As

In which types of tissues is EpCAM predominantly expressed?

EpCAM is highly expressed in epithelial tissues and some cancer cells.

How does EpCAM contribute to cell adhesion?

It has a controversial role in cell adhesion, possibly contributing to both cell-cell adhesion and anti-adhesive properties.

What is the primary function of EpCAM in cells?

EpCAM primarily functions in cell signaling, proliferation, and migration.

What role does EpCAM play in cancer progression?

It often has a pro-tumorigenic role, promoting cancer cell proliferation, migration, and invasion.

How is EpCAM used as a biomarker in cancer diagnosis?

EpCAM is used as a biomarker to detect and isolate circulating tumor cells.

What therapeutic strategies are being explored involving EpCAM?

Strategies include developing EpCAM-targeted therapies for cancer treatment.

Are there known genetic alterations in EpCAM associated with specific health conditions?

Genetic alterations in EpCAM are linked to some hereditary cancer syndromes.