EPHA2-541H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human EPHA2 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=47kDa.
Description :
EPHA2 is a member of the ephrin receptor subfamily of protein-tyrosine kinases that bind the ephrin-A ligand and have diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.
Source :
Sf9 insect cells.
Purity :
>90%.
Specific Activity :
170 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 0.1 mM ATP, 30 μg/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 μg /ml recombinant EPHB2.
Application :
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.