Recombinant Human EPH Receptor A2, His-tagged

• Cat.No.: EPHA2-541H
• Product Overview: Recombinant human EPHA2 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=47kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: EPHA2 is a member of the ephrin receptor subfamily of protein-tyrosine kinases that bind the ephrin-A ligand and have diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.
• Purity: >90%.
• Specific Activity: 170 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 0.1 mM ATP, 30 μg/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 μg /ml recombinant EPHB2.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 50% glycerol and 3mM DTT.
• Stability: >12 months at –80°C.

Gene Information

• Gene Name: EPHA2 EPH receptor A2 [ Homo sapiens ]
• Synonyms: EPHA2; EPH receptor A2; ECK; ARCC2; protein tyrosine kinase; soluble EPHA2 variant 1; soluble EPHA2 variant 1; epithelial cell receptor protein tyrosine kinase; ephrin receptor EphA2; EC 2.7.10.1
• Gene ID: 1969
• mRNA Refseq: NM_004431
• Protein Refseq: NP_004422
• MIM: 176946
• UniProt ID: P29317
• Chromosome Location: 1p36
• Pathway: Axon guidance
• Function: ATP binding; ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transferase activity