Active Recombinant Human EPHA10 protein, His-tagged, Low Endotoxin

• Cat.No.: EPHA10-7844H
• Product Overview: Recombinant Human EPHA10 protein(Q5JZY3-1)(Glu34-Ala565), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 34-565 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EPHA10, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EPHA10 EPH receptor A10 [ Homo sapiens ]
• Official Symbol: EPHA10
• Synonyms: EPHA10; EPH receptor A10; ephrin type-A receptor 10; FLJ16103; FLJ33655; EphA10s protein; MGC43817
• Gene ID: 284656
• mRNA Refseq: NM_001099439
• Protein Refseq: NP_001092909
• MIM: 611123
• UniProt ID: Q5JZY3