Recombinant Human EPHA8 Protein, GST-tagged
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| Cat.No. : | EPHA8-3396H |
| Product Overview : | Human EPHA8 (NP_065387.1, 571 a.a. - 924 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. |
| Description : | This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein encoded by this gene functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq, Jul 2008] |
| Source : | Insect cells |
| Species : | Human |
| Tag : | GST |
| Form : | Liquid |
| Bio-activity : | The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Blk/Lyntide. ATP: 100 μM. |
| Molecular Mass : | 67 kDa |
| Purity : | 89 % by SDS-PAGE/CBB staining |
| Applications : | Functional Study SDS-PAGE |
| Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : | In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol) |
| Quality Control Testing : |
Loading 1 μg protein in SDS-PAGE |
| Result of activity analysis : |
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| Gene Name : | EPHA8 EPH receptor A8 [ Homo sapiens ] |
| Official Symbol : | EPHA8 |
| Synonyms : | EPHA8; EPH receptor A8; EEK, EphA8; ephrin type-A receptor 8; Hek3; EPH-like kinase 3; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; tyrosine-protein kinase receptor EEK; EEK; EK3; HEK3; KIAA1459; |
| Gene ID : | 2046 |
| mRNA Refseq : | NM_001006943 |
| Protein Refseq : | NP_001006944 |
| MIM : | 176945 |
| UniProt ID : | P29322 |
◆ Recombinant Protein
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