| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
1-250 aa |
| Form : |
Lyophilized from sterile PBS, 8% sucrose, 0.5% Tween-20, pH 7.4. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized CD131 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human EPOR with a linear range of 0.16-4 μg/ml.
2. Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 4-16 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO. |
| Molecular Mass : |
The recombinant human EPOR/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 460 amino acids and has a predicted molecular mass of 51.0 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEPOR/Fc monomer is approximately 55-60 kDa due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 90 % as determined by SDS-PAGE |
| Storage : |
Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
