Active Recombinant Human EPOR Protein, Fc-tagged

• Cat.No.: EPOR-29H
• Product Overview: Recombinant Human EPOR Protein (Ala 25 - Cys 241) (AAI12154), fused with a human IgG1 Fc tag at the C-terminus, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Ala 25 - Cys 241
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human EPO, Tag Free at 10μg/mL (100 μL/well) can bind Human EPO R, Fc Tag with a linear range of 0.013-0.12 μg/mL.
• Molecular Mass: Recombinant Human EPOR Protein has a calculated MW of 50.1 kDa. The protein migrates as 55-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]
• Official Symbol: EPOR
• Synonyms: EPOR; erythropoietin receptor; EPO-R; MGC138358
• Gene ID: 2057
• mRNA Refseq: NM_000121
• Protein Refseq: NP_000112
• MIM: 133171
• UniProt ID: P19235
• Chromosome Location: 19p13.3-p13.2
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem
• Function: erythropoietin receptor activity; identical protein binding; protein binding; receptor activity