Recombinant Human ERI1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ERI1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ERI1-3313H
Product Overview : ERI1 MS Standard C13 and N15-labeled recombinant protein (NP_699163) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 40.1 kDa
AA Sequence : MEDPQSKEPA GEAVALALLE SPRPEGGEEP PRPSPEETQQ CKFDGQETKG SKFITSSASD FSDPVYKEIA ITNGCINRMS KEELRAKLSE FKLETRGVKD VLKKRLKNYY KKQKLMLKES NFADSYYDYI CIIDFEATCE EGNPPEFVHE IIEFPVVLLN THTLEIEDTF QQYVRPEINT QLSDFCISLT GITQDQVDRA DTFPQVLKKV IDWMKLKELG TKYKYSLLTD GSWDMSKFLN IQCQLSRLKY PPFAKKWINI RKSYGNFYKV PRSQTKLTIM LEKLGMDYDG RPHCGLDDSK NIARIAVRML QDGCELRINE KMHAGQLMSV SSSLPIEGTP PPQMPHFRKT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ERI1 exoribonuclease 1 [ Homo sapiens (human) ]
Official Symbol : ERI1
Synonyms : ERI1; exoribonuclease 1; THEX1, three prime histone mRNA exonuclease 1; 3-5 exoribonuclease 1; 3HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1 (C.elegans); Eri-1 homolog; protein 3hExo; 3 exoribonuclease; 3-5 exonuclease ERI1; histone mRNA 3-exonuclease 1; three prime mRNA exonuclease 1; three prime histone mRNA exonuclease 1; histone mRNA 3 end-specific exonuclease; histone mRNA 3-end-specific exoribonuclease; enhanced RNAi three prime mRNA exonuclease homolog 1; HEXO; THEX1; MGC35395;
Gene ID : 90459
mRNA Refseq : NM_153332
Protein Refseq : NP_699163
MIM : 608739
UniProt ID : Q8IV48

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