Recombinant Human Estrogen Receptor 1

• Cat.No.: ESR1-116H
• Product Overview: Recombinant Human Estrogen Receptor protein expressed inE.Coliencodes 1-300 amino acids and has a molecular mass of 59 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-300 a.a.
• Description: The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
• Formulation: 2µg/20µl Recombinant Human ER α protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application: •ELISA •Inhibition Assays •Western Blotting.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 59 kDa.
• Storage & Stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.

Gene Information

• Gene Name: ESR1 estrogen receptor 1 [ Homo sapiens ]
• Synonyms: ESR1; estrogen receptor 1; ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123;Estrogen receptor;ER-alpha; Nuclear receptor subfamily 3 group A member 1
• Gene ID: 2099
• mRNA Refseq: NM_000125
• Protein Refseq: NP_000116
• UniProt ID: P03372
• Chromosome Location: 6q24-q27
• MIM: 133430
• Function: estrogen receptor activity; estrogen response element binding; hormone binding; metal ion binding; nitric-oxide synthase regulator activity; protein N-terminus binding; sequence-specific DNA binding; steroid binding; transcription factor activity; zinc ion binding

Citation

Ketamine and Ketamine Metabolites as Novel Estrogen Receptor Ligands: Induction of Cytochrome P450 and AMPA Glutamate Receptor Gene Expression

Journal: Biochemical pharmacology    PubMed ID: 29621538    Data: 2019/6/1

Authors: Ming-Fen Ho, Cristina Correia, Richard M. Weinshilboum

Article Snippet:Recombinant full length human estrogen receptor alpha (ESR1-1033H, Creative BioMart, NY, USA) was used to perform radioligand binding assays and surface plasmon resonance (SPR).. For the radioligand binding assays, protein was diluted in ice-cold assay buffer (10 mM Tris-HCl; 1 mM EDTA; 1 mM EGTA; 1 mM NaVO3; 1% glycerol; 0.25 mM leupeptin; 1% BSA; 1 mM DTT).For the radioligand binding assays, protein was diluted in ice-cold assay buffer (10 mM Tris-HCl; 1 mM EDTA; 1 mM EGTA; 1 mM NaVO3; 1% glycerol; 0.25 mM leupeptin; 1% BSA; 1 mM DTT).

Primer sequences