"EZH2" Related Products

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Recombinant Human EZH2 protein, His-tagged

Cat.No.: EZH2-175H
Product Overview: Mutant version of EZH2 5-member complex, but with a Tyr-to-Phe mutation on a.a. 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end; Y646F*, with N-terminal His tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.
Source: Insect cells
Species: Human
Tag: His
Form: 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, and 10% glycerol.
Molecular Mass: 325 kDa (complex)
Protein length: 2–end (all components)
Purity: ≥ 91%
Applications: Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
Storage: >6 months at -80 centigrade. Avoid freeze/thaw cycles
Concentration: 0.25 mg/ml
Gene Name: EZH2 enhancer of zeste homolog 2 (Drosophila) [ Homo sapiens ]
Official Symbol: EZH2
Synonyms: EZH2; enhancer of zeste homolog 2 (Drosophila); enhancer of zeste (Drosophila) homolog 2; histone-lysine N-methyltransferase EZH2; ENX 1; EZH1; KMT6; KMT6A; lysine N-methyltransferase 6; ENX1; WVS2; ENX-1; MGC9169;
Gene ID: 2146
mRNA Refseq: NM_004456
Protein Refseq: NP_004447
MIM: 601573
UniProt ID: Q15910
Chromosome Location: 7q35-q36
Function: DNA binding; histone methyltransferase activity; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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