||Recombinant Human EZR fused with GST tag at the N-terminus was produced in E. coli.
||Ezrin is probably participating in connecting the cytoskeleton to the plasma membrane. Like many proteins, Ezrin is autoinhibited by a head-tail interaction.
||100mM KCl, 20mM Hepes pH 7.4, 0.2mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution.
||Purity: >96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Purification notes: Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.
||Ezrin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect. Avoid repeated freeze / thaw cycles.
||At ambient temperature. Upon delivery store at -70 centigrade.