Recombinant Human F12 protein, His & T7-tagged
Cat.No. : | F12-773H |
Product Overview : | Recombinant Human F12 aa. (Ser400~Ser615 (Accession # P00748)) fused with N-terminal His & T7 tag was produced in E. coli cells. |
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Description : | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. |
Source : | E. coli |
Species : | Human |
Tag : | His&T7 |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 27.2kDa |
Protein length : | Ser400~Ser615 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | >95% |
Characteristic : | The isoelectric point is 5.5. |
Applications : | SDS-PAGE; WB; ELISA; IP. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Gene Name : | F12 coagulation factor XII [ Homo sapiens (human) ] |
Official Symbol : | F12 |
Synonyms : | HAF; HAE3; HAEX; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; coagulation factor XII |
Gene ID : | 2161 |
mRNA Refseq : | NM_000505.3 |
Protein Refseq : | NP_000496.2 |
UniProt ID : | P00748 |
Products Types
◆ Recombinant Protein | ||
F12-2040P | Recombinant Pig F12 Protein (20-371 aa), His-tagged | +Inquiry |
F12-2005P | Recombinant Pig F12 Protein (20-371 aa), His-tagged | +Inquiry |
F12-3606H | Recombinant Human F12 Protein, GST-tagged | +Inquiry |
F12-909M | Recombinant Mouse F12 Protein, His-tagged | +Inquiry |
F12-1833R | Recombinant Rat F12 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
F12-13H | Native Human Factor beta-XIIa, Biotin Labeled | +Inquiry |
F12-5397H | Active Native Human Coagulation Factor XII (Hageman factor) | +Inquiry |
F12-28805TH | Native Human F12 | +Inquiry |
◆ Lysates | ||
F12-2115HCL | Recombinant Human F12 cell lysate | +Inquiry |
Related Gene
Not For Human Consumption!
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Write a reviewTrustworthy data source. Valuable for our experiments.
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Enables data-driven decisions. A research necessity.
Q&As (7)
Ask a questionAbnormalities in F12 can lead to increased risk of thrombosis and related disorders.
Genetic mutations in F12 can result in either excessive bleeding or an increased tendency to form clots.
F12 works in conjunction with other coagulation factors and proteins to regulate clot formation and breakdown.
Altered F12 activity is associated with inflammation and can influence immune responses.
Targeting F12 offers potential in treating clotting disorders and reducing inflammation in certain conditions.
Upon activation, F12 triggers a series of enzymatic reactions leading to clot formation.
Factor XII (F12) activates the intrinsic pathway of the coagulation cascade, crucial for blood clot formation.
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