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Recombinant Human F12 protein, His & T7-tagged

Cat.No. : F12-773H
Product Overview : Recombinant Human F12 aa. (Ser400~Ser615 (Accession # P00748)) fused with N-terminal His & T7 tag was produced in E. coli cells.
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Description : This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.
Source : E. coli
Species : Human
Tag : His&T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 27.2kDa
Protein length : Ser400~Ser615
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : F12 coagulation factor XII [ Homo sapiens (human) ]
Official Symbol : F12
Synonyms : HAF; HAE3; HAEX; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; coagulation factor XII
Gene ID : 2161
mRNA Refseq : NM_000505.3
Protein Refseq : NP_000496.2
UniProt ID : P00748

Not For Human Consumption!

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06/23/2023

    Trustworthy data source. Valuable for our experiments.

    05/22/2022

      Accelerates research progress. Exceptional service provider.

      03/26/2018

        Enables data-driven decisions. A research necessity.

        Q&As (7)

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        What role does F12 play in thrombotic disorders? 05/30/2023

        Abnormalities in F12 can lead to increased risk of thrombosis and related disorders.

        How do genetic variations in the F12 gene affect coagulation and risk of bleeding or clotting disorders? 03/26/2022

        Genetic mutations in F12 can result in either excessive bleeding or an increased tendency to form clots.

        How does F12 interact with other components of the coagulation and fibrinolytic systems? 10/01/2021

        F12 works in conjunction with other coagulation factors and proteins to regulate clot formation and breakdown.

        What is the impact of altered F12 activity on inflammation and immune response? 04/28/2021

        Altered F12 activity is associated with inflammation and can influence immune responses.

        What potential therapeutic applications could arise from targeting F12 in clotting or inflammatory disorders? 12/29/2019

        Targeting F12 offers potential in treating clotting disorders and reducing inflammation in certain conditions.

        How does F12 activation initiate the intrinsic pathway of coagulation? 10/05/2018

        Upon activation, F12 triggers a series of enzymatic reactions leading to clot formation.

        What is the primary function of Factor XII (F12) in the blood coagulation cascade? 03/23/2017

        Factor XII (F12) activates the intrinsic pathway of the coagulation cascade, crucial for blood clot formation.

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