Recombinant Human F7 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human F7 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : F7-530H
Product Overview : F7 MS Standard C13 and N15-labeled recombinant protein (NP_062562) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 49.3 kDa
AA Sequence : MVSQALRLLC LLLGLQGCLA AVFVTQEEAH GVLHRRRRAN AFLEELRPGS LERECKEEQC SFEEAREIFK DAERTKLFWI SYSDGDQCAS SPCQNGGSCK DQLQSYICFC LPAFEGRNCE THKDDQLICV NENGGCEQYC SDHTGTKRSC RCHEGYSLLA DGVSCTPTVE YPCGKIPILE KRNASKPQGR IVGGKVCPKG ECPWQVLLLV NGAQLCGGTL INTIWVVSAA HCFDKIKNWR NLIAVLGEHD LSEHDGDEQS RRVAQVIIPS TYVPGTTNHD IALLRLHQPV VLTDHVVPLC LPERTFSERT LAFVRFSLVS GWGQLLDRGA TALELMVLNV PRLMTQDCLQ QSRKVGDSPN ITEYMFCAGY SDGSKDSCKG DSGGPHATHY RGTWYLTGIV SWGQGCATVG HFGVYTRVSQ YIEWLQKLMR SEPRPGVLLR APFPSGPTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : F7 coagulation factor VII [ Homo sapiens (human) ]
Official Symbol : F7
Synonyms : F7; coagulation factor VII (serum prothrombin conversion accelerator); coagulation factor VII; eptacog alfa; factor VII; FVII coagulation protein; SPCA; proconvertin; serum prothrombin conversion accelerator;
Gene ID : 2155
mRNA Refseq : NM_019616
Protein Refseq : NP_062562
MIM : 613878
UniProt ID : P08709

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