Recombinant Human FAP, His-tagged

• Cat.No.: FAP-28897TH
• Product Overview: Recombinant fragment corresponding to amino acids 29-760 of Human Fibroblast activation protein, alpha with C terminal His tag expressed in a Baculovirus infected Sf9 cell expression system, 85.5 kDa.

Specification

• Description: The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
• Conjugation: HIS
• Tissue specificity: Fibroblast specific.
• Biological activity: Specific Activity: 10.2 pmol/min/μg.Assay Condition: 50 mM Tris-HCl (pH7.5), 10 mM MgCl2, 1 mM MnCl2, 5μM substrate (A-P-AMC), room temperature.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the peptidase S9B family.

Gene Information

• Gene Name: FAP fibroblast activation protein, alpha [ Homo sapiens ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase; DPPIV
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451
• MIM: 600403
• Uniprot ID: Q12884
• Chromosome Location: 2q23
• Function: dipeptidyl-peptidase activity; metalloendopeptidase activity; peptidase activity; protein binding; protein dimerization activity