Active Recombinant Human FAP protein, His-tagged

• Cat.No.: FAP-1141H
• Product Overview: Active Recombinant Human FAP protein(NP_004451.2)(Leu26-Asp760) is expressed in HEK293, fused with a 6xHis tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 26-760 aa
• Form: Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human FAP at 1 μg/mL (100 μL/well) can bind FAP Rabbit mAb with a linear range of 0.03-3.94 ng/mL. 2.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).The specific activity is >2863 pmol/min/μg. 3.Measured by its ability to hydrolyze the substrate Z-Gly-Pro-AMC to Z-Gly-Pro and AMC. The specific activity is >3000 pmol/min/μg.
• Molecular Mass: 85.87 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FAP fibroblast activation protein, alpha [ Homo sapiens ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451
• MIM: 600403
• UniProt ID: Q12884