Recombinant Mouse FAP protein, His-tagged

• Cat.No.: FAP-1443M
• Product Overview: Recombinant Mouse FAP protein(P97321-1)(Leu26-Asp761), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Leu26-Asp761
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse FAP, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Fap fibroblast activation protein [ Mus musculus ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein; seprase; integral membrane serine protease; fibroblast activation protein alpha
• Gene ID: 14089
• mRNA Refseq: NM_007986
• Protein Refseq: NP_032012