Active Recombinant Mouse Fap protein, His-tagged, Low Endotoxin

• Cat.No.: FAP-1443M
• Product Overview: Recombinant Mouse Fap protein(P97321-1)(26-761 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 26-761 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse FAP, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Fap fibroblast activation protein [ Mus musculus ]
• Official Symbol: Fap
• Synonyms: FAP; fibroblast activation protein; seprase; integral membrane serine protease; fibroblast activation protein alpha
• Gene ID: 14089
• mRNA Refseq: NM_007986
• Protein Refseq: NP_032012