Active Recombinant Human FAS protein, His-tagged
| Cat.No. : | FAS-614H |
| Product Overview : | Active Recombinant Human FAS protein(P25445)(26~173 aa), fused with N-terminal His tag, was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
- Download
| Species : | Human |
| Source : | E.coli |
| Tag : | His |
| Protein Length : | 26-173 aa |
| Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : | FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with FASL. Briefly, FASL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul FASL were then transferred to FAS-coated microtiter wells (1ug/ml, 100ul/well) and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FASL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and FASL was shown, and this effect was in a dose dependent manner, the EC50 was approximately 0.012 ug/mL. |
| Molecular Mass : | 17.9kDa |
| Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
| Purity : | > 95% as determined by SDS-PAGE. |
| Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
| Gene Name | FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ] |
| Official Symbol | FAS |
| Synonyms | FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; |
| Gene ID | 355 |
| mRNA Refseq | NM_000043 |
| Protein Refseq | NP_000034 |
| MIM | 134637 |
| UniProt ID | P25445 |
| ◆ Recombinant Proteins | ||
| FAS-37H | Recombinant Human Fas Ligand Inhibitor, Fc-tagged | +Inquiry |
| FAS-4270H | Recombinant Human Fas (TNF Receptor Superfamily, Member 6) | +Inquiry |
| FAS-921C | Recombinant Cattle FAS Protein, His&GST-tagged | +Inquiry |
| FAS-243H | Recombinant Human FAS Protein, Fc-tagged | +Inquiry |
| FAS-281H | Recombinant Human FAS, His & GST tagged | +Inquiry |
| ◆ Native Proteins | ||
| FAS-27H | Active Recombinant Human FAS Homodimer Protein, His tagged | +Inquiry |
| ◆ Cell & Tissue Lysates | ||
| FAS-1139RCL | Recombinant Rat FAS cell lysate | +Inquiry |
| FAS-2185HCL | Recombinant Human FAS cell lysate | +Inquiry |
| FAS-2419MCL | Recombinant Mouse FAS cell lysate | +Inquiry |
| FAS-001CCL | Recombinant Cynomolgus FAS cell lysate | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews (0)
- Q&As (0)
Ask a Question for All FAS Products
Required fields are marked with *
My Review for All FAS Products
Required fields are marked with *
