Active Recombinant Human FAS protein, His-tagged

Cat.No. : FAS-614H
Product Overview : Active Recombinant Human FAS protein(P25445)(26~173 aa), fused with N-terminal His tag, was expressed in E.coli.
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Species : Human
Source : E.coli
Tag : His
Protein Length : 26-173 aa
Form : Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Bio-activity : FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with FASL. Briefly, FASL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul FASL were then transferred to FAS-coated microtiter wells (1ug/ml, 100ul/well) and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FASL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and FASL was shown, and this effect was in a dose dependent manner, the EC50 was approximately 0.012 ug/mL.
Molecular Mass : 17.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
Official Symbol FAS
Synonyms FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6;
Gene ID 355
mRNA Refseq NM_000043
Protein Refseq NP_000034
MIM 134637
UniProt ID P25445

Not For Human Consumption!

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