"FCGR3B" Related Products

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Recombinant Human FCGR3B Protein, His-Avi-tagged, Biotinylated

Cat.No.: FCGR3B-354H
Product Overview: Biotinylated Human CD16b (NA2), His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # O75015). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source: HEK293
Species: Human
Tag : His-Avi
Predicted N Terminal: Gly 17
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Biotinylated Human CD16b (NA2), His Tag, Avi Tag on SA Chip can bind Rituximab with an affinity constant of 6.66 uM as determined in a SPR assay (Biacore T200). Immobilized Biotinylated Human CD16b (NA2), His Tag, Avi Tag on Series S Sensor Chip CAP via Biotin Capture can bind Rituximab with an affinity constant of 4.22 uM as determined in a SPR assay (Biacore T200).
Molecular Mass: The protein has a calculated MW of 24.5 kDa. The protein migrates as 37-55 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) [ Homo sapiens ]
Official Symbol: FCGR3B
Synonyms: FCGR3B; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16) , FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-B; CD16; CD16b; fc-gamma RIII; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); FCG3; FCGR3; FCR-10; FCRIII; FCRIIIb;
Gene ID: 2215
mRNA Refseq: NM_000570
Protein Refseq: NP_000561
MIM: 610665
UniProt ID: O75015

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