Recombinant Human Ferredoxin 1, T7-tagged

• Cat.No.: FDX1-483H
• Product Overview: Recombinant human Ferredoxin 1protein, fused to T7-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 15.0 kDa (139aa).

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450.
• Amino Acid Sequence: MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS.
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% Glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: FDX1 ferredoxin 1 [ Homo sapiens ]
• Synonyms: FDX1; ferredoxin 1; ADX; FDX; LOH11CR1D; adrenodoxin; mitochondrial adrenodoxin; Adrenodoxin, mitochondrial; Adrenal ferredoxin; Ferredoxin-1; Hepatoredoxin; mitochondrial adrenodoxin
• Gene ID: 2230
• mRNA Refseq: NM_004109
• Protein Refseq: NP_004100
• MIM: 103260
• UniProt ID: P10109
• Chromosome Location: 11q22.3
• Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; iron ion binding; metal ion binding