Active Recombinant Human FGF18 Protein, His-tagged

• Cat.No.: FGF18-504H
• Product Overview: Recombinant human FGF18 (O76093) (Met 1-Ala 207) was fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Glu 28
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 1.17 μg/mL.
• Molecular Mass: The secreted recombinant human FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.3 kDa. The apparent molecular mass of rh FGF18 is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: Met 1-Ala 207
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Storage: Store it under sterile conditions at -20°C~-70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGF18 fibroblast growth factor 18 [ Homo sapiens ]
• Official Symbol: FGF18
• Synonyms: FGF18; fibroblast growth factor 18; FGF 18; ZFGF5; FGF-18
• Gene ID: 8817
• mRNA Refseq: NM_003862.2
• Protein Refseq: NP_003853.1
• MIM: 603726
• UniProt ID: O76093