Active Recombinant Mouse Fgf18 Protein (172 aa)

• Cat.No.: Fgf18-417F
• Product Overview: Recombinant mouse Fibroblast Growth Factor 18 (rmFGF-18) produced in E. coli is a single non-glycosylated polypeptide chain containing of 172 amino acids. A fully biologically active molecule, rmFGF-18 has a molecular mass of 20.1 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Description: Fibroblast Growth Factor 18 (FGF-18) is a pleiotropic cytokine belonging to the heparin-binding FGF family, which has 23 different members. Structurally, FGF-18 is closely related to FGF-8 and FGF-17. Like other FGFs, FGF-18 can bind to different FGF receptors in vivo. FGF-18 is expressed in various tissues and has multiple functions: during long bone growth, FGF-18 is expressed in perichondrium and developing joints, and regulates bone formation by inhibiting chondrocyte proliferation and differentiation; FGF-18 knock-out mice survive embryonic development, but exhibit skeletal abnormalities and die in the early neonatal period. FGF-18 also induces ectopic cartilage formation in the lung, and alters the morphology of the pulmonary mesenchyma.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 0.5 μg/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2 × 10^3 units/mg.
• Molecular Mass: 20.1 kDa, observed by non-reducing SDS-PAGE.
• Protein Length: 172
• AA Sequence: EENVDFRIHVENQTRARDDVSRKQL RLYQLYSRTSGKHIQVLGRRISARG EDGDKYAQLLVETDTFGSQVRIKGK ETEFYLCMNRKGKLVGKPDGTSKEC VFIEKVLENNYTALMSAKYSGWYVG FTKKGRPRKGPKTRENQQDVHFMKR YPKGQAELQKPFKYTTVTKRSR
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Storage: Lyophilized recombinant mouse Fibroblast Growth Factor 18 (rmFGF-18) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmFGF-18 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: Fgf18 fibroblast growth factor 18 [ Mus musculus ]
• Official Symbol: Fgf18
• Synonyms: FGF18; fibroblast growth factor 18; zFGF5; FGF-18; D130055P09Rik
• Gene ID: 14172
• mRNA Refseq: NM_008005
• Protein Refseq: NP_032031
• UniProt ID: O89101

Reviews

01/09/2022

the FGF18 protein exhibits remarkable stability, enabling it to maintain its structural integrity and functionality throughout the experimental process.

05/30/2021

The FGF18 protein offers exceptional quality and purity, which is crucial for ensuring reliable and reproducible experimental outcomes.

12/08/2017

As a researcher utilizing the FGF18 protein, I am fortunate to benefit from its numerous advantages in trials.

Q&As

What are the potential side effects of FGF18 therapy?

While generally considered safe, potential side effects may include local reactions at the injection site. Comprehensive studies are ongoing to assess long-term safety.

Is FGF18 only applicable to bone-related conditions?

While its primary focus is on musculoskeletal conditions, FGF18's role in cellular growth and repair suggests potential applications in other medical fields.

How does FGF18 compare to other growth factors in terms of clinical applications?

Comparative studies are underway to evaluate the effectiveness and safety of FGF18 against other growth factors in various clinical scenarios.

Are there any ongoing clinical trials involving FGF18?

Yes, there are several clinical trials exploring the safety and efficacy of FGF18 in various musculoskeletal disorders and conditions.

How does FGF18 contribute to the treatment of osteoarthritis?

FGF18 has shown promise in promoting cartilage repair and regeneration, making it a potential candidate for the development of therapies for osteoarthritis.