Recombinant Human FGFR1, Fc Chimera

• Cat.No.: FGFR1-498H
• Product Overview: Recombinant Human Fibroblast Growth Factor Receptor 1 encoding the signal peptide and extracellular domain of human Fibroblast Growth Factor Receptor 1 alpha (IIIc) (aa 1-363) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

Specification

• Cat. No.: FGFR1-498H
• Description: Fibroblast growth factors (FGFs) belong to a large family (23 human members) of mitogenic factors that range in molecular mass from 17-34kDa. FGFs exhibit high affinity for heparin and heparin like glycosaminoglycans (HLGAGs), and these interactions are required for signaling activation from the FGF receptors.
• Source: Human 293 cells.
• Amino Acid Sequence: RPSPTLPEQAQPWGAPVEVESFLVH PGDLLQLRCRLRDDVQSINWLRDGV QLAESNRTRITGEEVEVQDSVPADS GLYACVTSSPSGSDTTYFSVNVSDA LPSSEDDDDDDDSSSEEKETDNTKP NRMPVAPYWTSPEKMEKKLHAVPAA KTVKFKCPSSGTPNPTLRWLKNGKE FKPDHRIGGYKVRYATWSIIMDSVV PSDKGNYTCIVENEYGSINHTYQLD VVERSPHRPILQAGLPANKTVALGS NVEFMCKVYSDPQPHIQWLKHIEVN GSKIGPDNLPYVQILKTAGVNTTDK EMEVLHLRNVSFEDAGEYTCLAGNS IGLSHHSAWLTVLEALEGIPKVDKK VEPKSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCRVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPEN NYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQK SLSLSPGK.
• Molecular Mass: FGF R1 alpha (IIIc)-Fc Chimera migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation.
• pI: Due to post-translational modifications, in particular glycosylation, the Symansis FGF R1 alpha (IIIc)-Fc Chimera separates into multiple isoforms with a pI between 5.2 and 6.9 in 2D PAGE.
• % Carbohydrate: Purified FGF R1 alpha (IIIc)-Fc Chimera consists of 35-50% carbohydrate (by wt).
• Glycosylation: FGF R1 alpha (IIIc)-Fc Chimera has N-linked and probably O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: FGFR1 fibroblast growth factor receptor 1 [ Homo sapiens ]
• Synonyms: FGFR1; fibroblast growth factor receptor 1; CEK; FLG; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; HBGFR; N-SAM; FLJ99988; OTTHUMP00000190881; soluble FGFR1 variant 1; FMS-like tyrosine kinase 2; ydroxyaryl-protein kinase; fms-related tyrosine kinase 2; heparin-binding growth factor receptor; basic fibroblast growth factor receptor 1
• Gene ID: 2260
• mRNA Refseq: NM_015850
• Protein Refseq: NP_056934
• UniProt ID: P11362
• Chromosome Location: 8p12
• MIM: 136350
• Pathway: Adherens junction; MAPK signaling pathway; elanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Axon guidance; Signaling by FGFR
• Function: ATP binding; fibroblast growth factor receptor activity; heparin binding; nucleotide binding; protein binding; transferase activity