Recombinant Human Fibroblast Growth Factor

• Cat.No.: FGFR2-102H
• Product Overview: Recombinant Human Fibroblast Growth Factor-2 (FGF-2) produced inSf9insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The FGF-basic is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.
• Physical Appearance: Sterile Filtered liquid formulation.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The sterile protein solution (0.5 mg/ml) contains 20 mM Tris pH=7.9, 100 mM KCl, 1 mM DTT and 20% glycerol.
• Specific Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106 Units/mg.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.
• Stability: Fibroblast Growth Factor-basic although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: FGFR2 fibroblast growth factor receptor 2 [ Homo sapiens ]
• Synonyms: FGFR2; fibroblast growth factor receptor 2; BEK; JWS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM; FLJ98662; FGFR2; KSAR; FGFR-2; KSAM; OTTHUMP00000020621; OTTHUMP00000020629; BEK fibroblast growth factor receptor; CD332 antigen; FGF receptor; Jackson-Weiss syndrome; Keratinocyte growth factor receptor 2; bacteria-expressed kinase; craniofacial dysostosis 1; fibroblast growth factor receptor 2; hydroxyaryl-protein kinase; keratinocyte growth factor receptor; protein tyrosine kinase, receptor like 14; soluble FGFR4 variant 4
• Gene ID: 2263
• mRNA Refseq: NM_000141
• Protein Refseq: NP_000132
• UniProt ID: P21802
• Chromosome Location: 10q26
• MIM: 176943
• Pathway: Endocytosis; MAPK signaling pathway; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Signaling by FGFR
• Function: ATP binding; fibroblast growth factor receptor activity; heparin binding; nucleotide binding; protein binding; receptor activity; transferase activity