Recombinant Human FIP1L1 Protein, GST-tagged

• Cat.No.: FIP1L1-4177H
• Product Overview: Human FIP1L1 full-length ORF ( AAH17724, 1 a.a. - 378 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3 end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 67.32 kDa
• AA Sequence: MSAGEVERLVSELSGGTGGDEEEEW LYGDENEVERPEEENASANPPSGIE DETAENGVPKPKVTETEDDSDSDSD DDEDDVHVTIGDIKTGAPQYGSYGT APVNLNIKTGGRVYGTTGTKVKGVD LDAPGSINGVPLLEVDLDSFEDKPW RKPGADLSDYFNYGFNEDTWKAYCE KQKRIRMGLEVIPVTSTTNKITAED CTMEVTPGAEIQDGRFNLFKVQQGR TGNSEKETALPSTKAEFTSPPSLFK TGLPPSRNSTSSQSQTSTASRKANS SVGKWQDRYGRAESPDLRRLPGAID VIGQTITISRVEGRRRANENSNIQV LSERSATEVDNNFSKPPPFFPPGAP PTHLPPPPFLPPPPTVSTAPPLIPP PGK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: FIP1L1 FIP1 like 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: FIP1L1
• Synonyms: FIP1L1; FIP1 like 1 (S. cerevisiae); pre-mRNA 3-end-processing factor FIP1; DKFZp586K0717; FIP1-like 1 protein; factor interacting with PAP; rearranged in hypereosinophilia; Rhe; hFip1; FLJ33619
• Gene ID: 81608
• mRNA Refseq: NM_001134937
• Protein Refseq: NP_001128409
• MIM: 607686
• UniProt ID: Q6UN15