Recombinant human FLT3, Active

• Cat.No.: FLT3-1550H
• Product Overview: Recombinant human FLt3 is a polypeptide chain containing 165 amino acids (27–181 of P49771-1 FLT3L_HUMAN). It has a predicted molecular mass of 18.4 kDa, however as result of potential glycosylation, the recombinant protein could migrate with an apparent molecular mass of 18-25 kDa in SDS-PAGE.

Specification

• Description: FLt3 ligand is a transmembrane glycoprotein that activates the FLt3 receptor (CD135, FLK2). FLT3 ligand, in synergy with other cytokines, stimulates the growth and differentiation of a number of important cell populations. Activation of FLt3 stimulates the growth and differentiation of very early stem cells involved in haematopoiesis, myelopoiesis, erythropoiesis and megakaryopoiesis. FLt3 activation mobilizes hematopoietic progenitor cells into peripheral blood and induces proliferation of myeloid and dendritic cells (DC). Dysregulation of FLt3 has been associated with acute leukaemia(s) involving myeloid (AML), T, and B blast cell(s).
• Source: Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product.
• Species: Human
• Form: Recombinant human Flt3 is lyophilized from a Tris HCl 0.05M buffer pH 7.4.
• Molecular Mass: 18.4 kDa, apparent molecular mass of 18-25 kDa in SDS-PAGE.
• AA Sequence: HHHHHHTQDCSFQHSPISSDFAVKI RELSDYLLQDYPVTVASNLQDEELC GGLWRLVLAQRWMERLKTVAGSKMQ GLLERVNTEIHFVTKCAFQPPPSCL RFVQTNISRLLQETSEQLVALKPWI TRQNFSRCLELQCQPDSSTLPPPWS PRPLEATAPTA
• Endotoxin: < 0.04 eu/μg protein (lal>
• Purity: >97% by SDS-PAGE gel
• Storage: This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.
• Reconstitution: Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Optimal concentration should be determined for specific application and cell lines.
• Tag: Non
• Protein length: 27-181

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: ATP binding; cytokine receptor activity; nucleotide binding; phosphatidylinositol 3-kinase binding; protein homodimerization activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kina

Reviews

08/01/2021

Excellent product, great price.

12/22/2020

Satisfied, will order again.

09/14/2020

Quick response, helpful service.

Q&As

How is FLT3 implicated in acute myeloid leukemia (AML)?

FLT3 is frequently mutated in AML, contributing to uncontrolled cell growth and poor prognosis.

In which cells or tissues is FLT3 predominantly expressed?

FLT3 is predominantly expressed in hematopoietic stem cells and progenitor cells.

How does FLT3 contribute to hematopoiesis?

It is essential for hematopoiesis, particularly in the proliferation of early progenitor cells.

Are there therapeutic strategies targeting FLT3 for disease treatment?

Therapeutic strategies include FLT3 inhibitors, showing effectiveness in treating AML patients with FLT3 mutations.

What is the primary biological function of FLT3 in cells?

FLT3 is a receptor tyrosine kinase that plays a crucial role in cell survival, proliferation, and differentiation.

What role does FLT3 play in the proliferation and differentiation of hematopoietic stem cells?

It stimulates the proliferation and differentiation of hematopoietic stem cells, guiding their development into various blood cell types.

What are the common mutations found in FLT3 associated with diseases?

Common mutations include internal tandem duplications (ITD) and tyrosine kinase domain (TKD) mutations, leading to constitutive activation of FLT3.