Recombinant Human FLT3LG protein, N-His-tagged FLT3LG-157H

Recombinant Human FLT3LG protein, N-His-tagged

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Recombinant Human FLT3LG protein, N-His-tagged

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Cat.No. : FLT3LG-157H
Product Overview : Recombinant Human FLT3LG protein (Thr27-Pro185) was fused to His-tag at the N-terminus and expressed in Baculovirus-Insect cells.
Description : FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD13 -positive tissue counterparts.
Source : Baculovirus-Insect cells
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
Protein length : Thr27-Pro185
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 95% as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C.
Storage : Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
SDS-PAGE of FLT3LG-157H :

Activity of FLT3LG-157H :

Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 2-11 ng/mL.

Gene Name : FLT3LG
Official Symbol : FLT3LG
Synonyms : FL; FLG3L; FLT3L
Gene ID : 2323
mRNA Refseq : NM_001204502.2
Protein Refseq : NP_001191431.1
MIM : 600007
UniProt ID : P49771

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