|Product Overview :||Biotinylated Human VEGF R3, His Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # P35916-1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.|
|Description :||Vascular endothelial growth factor receptor 3 (VEGF R3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGF R3 mediates lymphangiogenesis in response to VEGF-C and VEGF-D. VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.|
|Tag :||His & Avi|
|Predicted N Terminal :||Tyr 25|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 88.2 kDa. The protein migrates as 115-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Tyr 25 - Ile 776|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
Biotinylated Human VEGF R3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||FLT4 fms related tyrosine kinase 4 [ Homo sapiens (human) ]|
|Official Symbol :||FLT4|
|Synonyms :||PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3; fms-like tyrosine kinase 4; tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3|
|Gene ID :||2324|
|mRNA Refseq :||NM_001354989.1|
|Protein Refseq :||NP_001341918.1|
|UniProt ID :||P35916|