Active Recombinant Human FNDC5 protein, His-tagged

• Cat.No.: FNDC5-542H
• Product Overview: Active Recombinant Human FNDC5 protein(2-137 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-137 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. FNDC5 is a 12 kDa glycosylated polypeptide hormone that regulate glycolipid metabolism, cardiovascular homeostasis metabolism, stem cell differentiation, and neuronal development. Myostatin (MSTN) has been identified as an interactor of FNDC5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FNDC5 and recombinant human MSTN. Briefly, FNDC5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MSTN-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FNDC5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FNDC5 and recombinant human MSTN was shown in Figure, the EC50 for this effect is 0.03 ug/mL.
• Molecular Mass: 19.0kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >85%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FNDC5 fibronectin type III domain containing 5 [ Homo sapiens ]
• Official Symbol: FNDC5
• Synonyms: FNDC5; fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FRCP2; fibronectin type III repeat-containing protein 2
• Gene ID: 252995
• mRNA Refseq: NM_001171940
• Protein Refseq: NP_001165411
• MIM: 611906
• UniProt ID: Q8NAU1