Active Recombinant Human FURIN Protein

• Cat.No.: FURIN-4553H
• Product Overview: Human FURIN (P09958) partial recombinant protein expressed in Hi-5 Insect cells.

Specification

• Description: The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. Some of its substrates are: proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene is thought to play a role in tumor progression. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq
• Source: Insect Cell
• Species: Human
• Form: Lyophilized
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-Arg-Val-Arg-Arg-AMC.
• Molecular Mass: 61.7 kDa
• AA Sequence: DLNVKAAWAQGYTGHGIVVSILDDG IEKNHPDLAGNYDPGASFDVNDQDP DPQPRYTQMNDNRHGTRCAGEVAAV ANNGVCGVGVAYNARIGGVRMLDGE VTDAVEARSLGLNPNHIHIYSASWG PEDDGKTVDGPARLAEEAFFRGVSQ GRGGLGSIFVWASGNGGREHDSCNC DGYTNSIYTLSISSATQFGNVPWYS EACSSTLATTYSSGNQNEKQIVTTD LRQKCTESHTGTSASAPLAAGIIAL TLEANKNLTWRDMQHLVVQTSKPAH LNANDWATNGVGRKVSHSYGYGLLD AGAMVALAQNWTTVAPQRKIIDILT EPKDIGKRLEVRKTVTACLGEPNHI TRLEHAQARLTLSYNRRGDLAIHLV SPMGTRSTLLAARPHDYSADGFNDW AFMTTHSWDEDPSGEWVLEIENTSE ANNYGTLTKFTLVLYGTAPEGLPVP PESSGCKTLTSSQACVVCEEGFSLH QKSCVQHCPPGFAPQVLDTHYSTEN DVETIRASVCAPCHACSATCQGPAL TDCLSCPSHASLDPVEQTCSRQSQS SRESPPQQQPPRLPPEVEAGQRLRA GLLPSHLPEHHHHHHHH
• Endotoxin: Endotoxin level is < 0.1 ng/μg (< 1 EU/μg).
• Purity: 95%
• Applications: Functional Study
  SDS-PAGE
• Usage: Activity assay
  SDS-PAGE
  The optimal working dilution should be determined by the end user.
• Storage: Store at -20 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: Lyophilized from solutions contain no sodiun azide nor carrier protein

Gene Information

• Gene Name: FURIN furin (paired basic amino acid cleaving enzyme) [ Homo sapiens ]
• Official Symbol: FURIN
• Synonyms: FURIN; furin (paired basic amino acid cleaving enzyme); FUR, PACE, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein), PCSK3; furin; SPC1; FES upstream region; dibasic processing enzyme; dibasic-processing enzyme; furin, membrane associated receptor protein; proprotein convertase subtilisin/kexin type 3; paired basic amino acid residue-cleaving enzyme; FUR; PACE; PCSK3
• Gene ID: 5045
• mRNA Refseq: NM_002569
• Protein Refseq: NP_002560
• MIM: 136950
• UniProt ID: P09958