Recombinant Human GABRA4 protein, His-tagged

• Cat.No.: GABRA4-7543H
• Product Overview: Recombinant Human GABRA4 protein(341-523 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 341-523 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: NYFTNIQMEKAKRKTSKPPQEVPAAPVQREKHPEAPLQNTNANLNMRKRTNALVHSESDVGNRTEVGNHSSKSSTVVQESSKGTPRSYLASSPNPFSRANAAETISAARALPSASPTSIRTGYMPRKASVGSASTRHVFGSRLQRIKTTVNTIGATGKLSATPPPSAPPPSGSGTSKIDKYAR
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: GABRA4 gamma-aminobutyric acid (GABA) A receptor, alpha 4 [ Homo sapiens ]
• Official Symbol: GABRA4
• Synonyms: GABRA4; gamma-aminobutyric acid (GABA) A receptor, alpha 4; gamma-aminobutyric acid receptor subunit alpha-4; GABA(A) receptor; alpha 4; GABA(A) receptor, alpha 4
• Gene ID: 2557
• mRNA Refseq: NM_000809
• Protein Refseq: NP_000800
• MIM: 137141
• UniProt ID: P48169