Description : |
GATM (Glycine amidinotransferase, mitochondrial) is a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. It plays a role in embryonic and central nervous system development. Recombinant human GATM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Source : |
E.coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl |
Molecular Mass : |
46.9 kDa |
Protein length : |
410 |
AA Sequence : |
MGSSHHHHHHSSGLVPRGSHMGSMS TQAATASSRNSCAADDKATEPLPKD CPVSSYNEWDPLEEVIVGRAENACV PPFTIEVKANTYEKYWPFYQKQGGH YFPKDHLKKAVAEIEEMCNILKTEG VTVRRPDPIDWSLKYKTPDFESTGL YSAMPRDILIVVGNEIIEAPMAWRS RFFEYRAYRSIIKDYFHRGAKWTTA PKPTMADELYNQDYPIHSVEDRHKL AAQGKFVTTEFEPCFDAADFIRAGR DIFAQRSQVTNYLGIEWMRRHLAPD YRVHIISFKDPNPMHIDATFNIIGP GIVLSNPDRPCHQIDLFKKAGWTII TPPTPIIPDDHPLWMSSKWLSMNVL MLDEKRVMVDANEVPIQKMFEKLGI TTIKVNIRNANSLGGGFHCWTCDVR RRGTLQSYLD |
Purity : |
>90% by SDS-PAGE |
Applications : |
SDS-PAGE |
Storage : |
Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
1mg/ml (determined by Bradford assay) |